Device

Part:BBa_K2326003:Design

Designed by: Zhongxiu Hu, Zhuoyi Yang, Xilin Yang   Group: iGEM17_BNDS_China   (2017-10-23)


LacI - pTac - gadR - gadC - gadA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3863
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3586
    Illegal AgeI site found at 3665
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4973
    Illegal SapI site found at 2505


Design Notes

We used the RBS calculator from Sallis Lab to calculate the appropriate RBS for gad R, C, A, and we used the translation rate ratio of 1:1:13, respectively.


Source

Lactobacillus brevis NCL912

References